摘要： 為了探尋重金屬銅對胡蘿卜種子萌發及幼苗生長的影響,將胡蘿卜種子置于不同濃度的CuSO4環境中,測定胡蘿卜種子發芽率,以及幼苗的根長、株高、鮮重與干重.結果 表明,低濃度的銅可以促進胡蘿卜種子萌發,促進幼苗根與地上部分生長,提高幼苗鮮重與干重;當CuSO4濃度達到150 mg/L,根的生長被抑制;當CuSO4濃度達到200mg/L,胡蘿卜種子的萌發被抑制;當CuSO4濃度達到250mg/L,幼苗的株高、干重與鮮重都顯著低于不合銅的組別.低濃度的銅可促進胡蘿卜生長發育,高濃度的銅會抑制胡蘿卜的生長發育.

摘要： 1 實驗裝置將Zn片浸在0.1 mol/L ZnSO4溶液中組成鋅半電池;將Cu片浸在0.1 mol/L CuSO4 溶液中組成銅半電池。取一縷脫脂棉條浸吸飽和KCl溶液后作鹽橋,組成銅-鋅原電池:(-)Zn(s)∣ZnSO4(0.1 mol/L )CuSO4(0.1 mol/L)Cu(s)(+)。2 實驗過程與討論2.1 測試原電池的基本性能分別測試瓊脂鹽橋、脫脂棉鹽橋原電池的電壓、電流及放電時長。數據見表1。

摘要： 硫酸銅(CuSO4)為白色粉末,硫酸銅水溶液則顯藍色,從水溶液中結晶時,可生成藍色的五水硫酸銅晶體(CuSO4·5H2O,又稱膽礬)。

摘要： Objective To observe the influences of copper sulfate on related inflammatory markers such as nuclear factor-κB( NF-κB) of HepG2 cells, the cell lines of hepatocellular carcinoma( HCC) . Methods HepG2 cells were cultured by RPMI-1640 contained various concentrations of copper sulfate in vitro. MTT colorimetric assay was used to detect the growth inhibitory effect of copper sulfate on HepG2 cell. Western blot was used to estimate the effect of copper sulfate on changes of related inflammatory markers, for instance, NF-κBp65, interleukin-1β(IL-1β), interleukin -4 ( IL-4 ) , interleukin-5 ( IL-5 ) , interleukin-6 ( IL-6 ) and γ-interferon ( IFN-γ) . Results The growth of HepG2 cells was inhibited by copper sulfate in certain concentrations. The expression levels of NF-κB, IFN-γand IL-5 were reduced and IL-1β, IL-4 and IL-6 were up-regulated in HepG2 cells by copper sulfate with a dose-dependent manner. Conclusion Copper sulfate may inhibit the proliferation of HepG2 cells. And the inflammatory reaction in HepG2 cells can be suppressed by copper sulfate through lowering the levels of NF-κB, IFN-γ and IL-5 and increasing the levels of IL-1β, IL-4 and IL-6 .%目的 觀察CuSO4對肝細胞癌(HCC)細胞株HepG2細胞核因子-κB(NF-κB)等相關炎癥指標的影響.方法 用含不同濃度CuSO4的RPMI-1640培養液孵育HepG2細胞,四甲基偶氮唑鹽(MTT)比色法檢測其對細胞增殖的抑制作用;Western blot法檢測不同濃度的CuSO4對HepG2細胞炎癥指標NF-κBp65、白細胞介素-1β(IL-1β)、白細胞介素-4(IL-4)、白細胞介素-5(IL-5)、白細胞介素-6(IL-6)和γ干擾素(IFN-γ)等表達水平的影響.結果 CuSO4在一定濃度范圍內可抑制HepG2細胞的增殖;CuSO4呈一定濃度依賴性地下調HepG2細胞中NF-κB、IFN-γ和IL-5蛋白的表達,上調IL-1β、IL-4和IL-6蛋白的表達.結論 CuSO4能抑制HepG2細胞的增殖,并可通過下調NF-κBp65、IFN-γ和IL-5蛋白的表達并上調IL-1β、IL-4和IL-6蛋白的表達來抑制HepG2細胞的炎癥反應.

摘要： 將銅片放入CuSO4溶液中蒸煮,經過一段時間后,在其表面得到Cu2O薄膜.改變蒸煮時間,利用原子力顯微鏡(AFM)對不同蒸煮時間的Cu2O薄膜觀察,得到相應的Cu2O薄膜表面形貌圖.實驗發現,銅片在CuSO4溶液中的蒸煮時間越長,表面顆粒的尺寸越大,越致密.當蒸煮時間達到60min時,Cu2O晶粒尺寸最大可達到1μm.由光致發光圖譜發現,蒸煮時間越長,Cu2O帶隙越寬.

摘要： 為了建立寒地粳稻成熟胚的高頻再生體系,以寒地粳稻空育131和東農425為試驗材料進行成熟胚培養,研究CuSO4和烯效唑的單獨和組合使用對水稻成熟胚培養的影響.結果表明:在培養基中單獨添加15.0μmol/L的Cu2+或添加0.50 mg/L的烯效唑有助于寒地粳稻成熟胚的誘導繼代和分化;在誘導和分化培養基中同時添加CuSO4和烯效唑時,空育131在CuSO4濃度為20.0μmol/L且烯效唑濃度為1.00 mg/L時,愈傷組織的誘導率和分化率都達到最高;CuSO4濃度為10.0μ mol/L,烯效唑濃度為1.00 mg/L時,東農425愈傷組織的誘導率最高,CuSO4濃度為15.0μ mol/L,烯效唑濃度為1.00 mg/L時,東農425愈傷組織的分化率最高.

摘要： 陜農33是2012年審定的一個高產、穩產、強筋優質的小麥優良新品種.為了解陜農33組織培養特性,以科農199、H261為對照,研究陜農33未成熟胚愈傷組織的誘導和分化能力以及AgNO3、CuSO4對其組織培養的影響.結果表明:陜農33未成熟胚愈傷組織誘導率為98.11％、褐化率為2.48％、再生分化率為80.23％.差異顯著性分析顯示:陜農33與對照科農199(誘導率99.43％、褐化率2.25％、分化率71.18％)沒有顯著性差異(P＞0.05),但顯著(P＜0.05)高于H261(誘導率96.24％、褐化率7.81％、分化率62.24％);說明陜農33具有較好的組織再生能力.另外,在培養基中添加4.0 mg/L的AgNO3可以進一步提高愈傷組織誘導率至99.41％、分化率至82.35％,使褐化率降至1.68％;添加2.50 mg/L的CuSO4可以提高綠苗分化率至84.17％.

摘要： With the increase of focus on food safety,copper agents which have the characteristics of pollution-free,no re?sistance etc. are widely used in organic agricultural production. Whether the extensive application of copper agents will bring influence on the yield and quality of foods or not is noteworthy. To inspect this influence,the changes of seed ger?mination,seedling growth and vegetable quality of Ipomoea aquatic under CuSO4 processing will be assayed in this pa?per.Results showed that CuSO4 treatment not over 1 mM had no obvious effect on seed germination rate,but would re?duce seed germination quality. CuSO4 treatment not over 9 mM had no obvious effect on seedling growth,photosynthesis, yield and nutrient content. CuSO4 treatment would increase copper accumulation in I. aquatic seedlings,and copper would accumulate preferentially in roots. Copper content in leaves and stems in the treated I. aquatic seedlings would not exceed the national standard,but it would exceed the standard in roots when CuSO4 was not less than 3 mM. CuSO4 treatment would reduce the nitrate reductase activities which led to the nitrate accumulation to the severe pollution level in I. aquatic seedlings. This would reduce the vegetable quality and enhance the human cancer risk.%隨著人們對食品安全關注度的增加，具有無公害、不產生抗藥性等特點的銅制劑正被大量應用于有機農業生產中。銅制劑的大量施用是否真對食品品質無害值得關注。本文以空心菜的種子和幼苗為材料，分析了CuSO4處理下種子萌發和幼苗生長、品質等的變化。結果顯示，≤1 mM CuSO4的處理對種子萌發率影響不明顯，但會降低種子萌發質量；≤9 mM CuSO4的處理對空心菜幼苗的生長、光合、產量和營養含量影響不大；CuSO4處理會增加空心菜體內的銅積累，并將銅優先積累于根部；各處理組空心菜莖葉銅含量不超標，但≥3 mM CuSO4處理將會導致根部銅含量超標；CuSO4處理降低了空心菜體內的硝酸還原酶活性，促使硝酸鹽在空心菜體內累積，并達到重度污染水平，降低了蔬菜品質，提高了人類的患癌風險。