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首頁> 外文期刊>Biochimica et biophysica acta. Molecular basis of disease: BBA >Adhesion contact kinetics of HepG2 cells during hepatitis B virus replication: Involvement of SH3-binding motif in HBX
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Adhesion contact kinetics of HepG2 cells during hepatitis B virus replication: Involvement of SH3-binding motif in HBX

機譯：乙型肝炎病毒復制過程中HepG2細胞的粘附接觸動力學：HBX中的SH3結合基序的參與

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It has been shown that Hepatitis B virus (RBV) replication directly alters the expression of key cytoskeleton-associated proteins which play key roles in mechanochemical signal transduction. Nevertheless, little is known on the correlation between HBV replication and the subsequent adhesion mechanism of HBV-replicating cells. In this study, it is demonstrated that the lag time of adhesion contact evolution of HepG2 cells with HBV replication is significantly increased by two times compared to that of normal HepG2 cell on collagen coated substrate. During the initial 20 min of cell seeding, only diffuse forms of vinculin was detected in HBV replicating cells while vinculin-associated focal complexes were found in normal and control cells. Similar delay in cell adhesion in HBV-replicating cells was observed in cells transfected with HBX, the smallest HBV protein, suggesting its involvement in this cellular process. In addition, a proline rich region found in many SH3 binding proteins was identified in HBX. HBX was found to interact with the focal adhesion protein, vinexin-beta, through the SH3 binding. Furthermore, HepG2 cells with HBV replication showed evidence of cell rounding up, possibly resulting from cytoskeletal reorganizations associated with interaction between HBX and vinexin-beta. Taken together, our results suggest that HBX is involved in the cytoskeletal reorganization in response to HBV replication. (c) 2006 Elsevier B.V. All rights reserved.
機譯：已經顯示，乙型肝炎病毒（RBV）復制直接改變了關鍵的細胞骨架相關蛋白的表達，這些蛋白在機械化學信號轉導中起關鍵作用。然而，關于HBV復制與隨后的HBV復制細胞粘附機制之間的相關性知之甚少。在這項研究中，已證明與正常的HepG2細胞在膠原蛋白包被的基質上相比，具有HBV復制的HepG2細胞的黏附接觸進化滯后時間顯著增加了兩倍。在細胞接種的最初20分鐘內，在HBV復制細胞中僅檢測到擴散形式的紐蛋白，而在正常細胞和對照細胞中發現了與紐蛋白相關的局灶復合物。在用最小的HBV蛋白HBX轉染的細胞中，觀察到了HBV復制細胞中細胞粘附的相似延遲，表明其參與了該細胞過程。另外，在HBX中鑒定出在許多SH3結合蛋白中發現的富含脯氨酸的區域。發現HBX通過SH3結合與粘著斑蛋白vinexin-beta相互作用。此外，具有HBV復制的HepG2細胞顯示出細胞聚集的跡象，可能是由于與HBX和vinexin-beta相互作用相關的細胞骨架重組所致。兩者合計，我們的結果表明HBX參與了對HBV復制的細胞骨架重組。 （c）2006 Elsevier B.V.保留所有權利。

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