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Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness uniformity and stability testing

機譯：開發和評估與番茄抗病性相關的魯棒性分子標記以進行獨特性均勻性和穩定性測試

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Molecular markers linked to phenotypically important traits are of great interest especially when traits are difficult and/or costly to be observed. In tomato where a strong focus on resistance breeding has led to the introgression of several resistance genes, resistance traits have become important characteristics in distinctness, uniformity and stability (DUS) testing for Plant Breeders Rights (PBR) applications. Evaluation of disease traits in biological assays is not always straightforward because assays are often influenced by environmental factors, and difficulties in scoring exist. In this study, we describe the development and/or evaluation of molecular marker assays for the Verticillium genes Ve1 and Ve2, the tomato mosaic virusTm1 (linked marker), the tomato mosaic virus Tm2 and Tm2² genes, the Meloidogyne incognita Mi1-2 gene, the Fusarium I (linked marker) and I2 loci, which are obligatory traits in PBR testing. The marker assays were evaluated for their robustness in a ring test and then evaluated in a set of varieties. Although in general, results between biological assays and marker assays gave highly correlated results, marker assays showed an advantage over biological tests in that the results were clearer, i.e., homozygote/heterozygote presence of the resistance gene can be detected and heterogeneity in seed lots can be identified readily. Within the UPOV framework for granting of PBR, the markers have the potential to fulfil the requirements needed for implementation in DUS testing of candidate varieties and could complement or may be an alternative to the pathogenesis tests that are carried out at present.Electronic supplementary materialThe online version of this article (doi:10.1007/s00122-009-1183-2) contains supplementary material, which is available to authorized users.

機譯：與表型上重要的性狀相關的分子標記引起了人們的極大興趣，尤其是當難以觀察和/或昂貴地觀察到性狀時。在對抗性育種的高度關注導致多個抗性基因滲入的番茄中，抗性狀已成為針對植物育種者權利（PBR）應用的獨特性，均勻性和穩定性（DUS）測試的重要特征。在生物檢測中評估疾病特征并不總是那么簡單，因為檢測通常受環境因素的影響，并且存在評分困難。在這項研究中，我們描述了黃萎病基因Ve1和Ve2，番茄花葉病毒Tm1（連鎖標記），番茄花葉病毒Tm2和Tm2 ^{2 基因的分子標記分析的開發和/或評估，根結線蟲Mi1-2基因，Fusarium I（連鎖標記）和I2基因座，它們是PBR檢測中的強制性狀。在環試驗中評估標記物測定的穩健性，然后在一組品種中進行評估。盡管一般而言，生物學測定和標記測定之間的結果給出了高度相關的結果，但標記測定顯示出優于生物學檢測的優勢，因為結果更清晰，即可以檢測到抗性基因的純合子/雜合子存在，并且可以檢測種子批次中的異質性。容易識別。在UPOV授予PBR的框架內，這些標記物有可能滿足DUS對候選品種進行測試所必需的要求，并且可以補充或可能替代目前進行的致病性測試。本文的版本（doi：10.1007 / s00122-009-1183-2）包含補充材料，可供授權用戶使用。}

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期刊名稱 Springer Open Choice
作者
Paul Arens; Carmen Mansilla; Dani?l Deinum; Laetitia Cavellini; André Moretti; Sophie Rolland; Hanneke van der Schoot; David Calvache; Fernando Ponz; Cécile Collonnier; René Mathis; Diederik Smilde; Carole Caranta; Ben Vosman;
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作者單位

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年(卷),期 -1(120),3
年度 -1
頁碼 655–664
總頁數 10
原文格式 PDF
正文語種
中圖分類外科學;
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1. Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing [J] . Arens Paul, Mansilla Carmen, Deinum Daniel, Theoretical and Applied Genetics: International Journal of Breeding Research and Cell Genetics . 2010,第3期

機譯：開發和評估與番茄抗病性相關的強大分子標記，以進行差異性，均勻性和穩定性測試
2. Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing [J] . Paul Arens, Carmen Mansilla, Dani?l Deinum, TAG Theoretical and Applied Genetics . 2010,第3期

機譯：開發和評估與番茄抗病性相關的魯棒性分子標記，以進行獨特性，均勻性和穩定性測試
3. The development of multiplex simple sequence repeat (SSR) markers to complement distinctness, uniformity and stability testing of rape (Brassica napus L.) varieties [J] . L. Tommasini, J. Batley, G. Arnold, Theoretical and Applied Genetics . 2003,第6期

機譯：多重簡單序列重復（SSR）標記的開發，以補充油菜（Brassica napus L.）品種的獨特性，均勻性和穩定性測試
4. Development of molecular markers linked to powdery mildew resistance genes in hop (Humulus lupulus L.) to support breeding for resistance [C] . R. Seidenberger, S. Mikolajewski, A. Lutz, European Brewery Convention Congress . 2007

機譯：開發與啤酒花白粉病抗性基因相關的分子標記，以支持抗性育種
5. Identification of molecular markers linked to disease resistance genes and applied marker-assisted breeding in wheat. [D] . Uphaus, James J. 2007

機譯：鑒定與抗病基因相關的分子標記，并在小麥中應用標記輔助育種。
6. Application of whole genome re-sequencing data in the development of diagnostic DNA markers tightly linked to a disease-resistance locus for marker-assisted selection in lupin (Lupinus angustifolius) [O] . Huaan Yang, Jianbo Jian, Xuan Li, 2015

機譯：全基因組重測序數據在診斷DNA標記開發中的應用該DNA標記與抗病基因座緊密相關用于羽扇豆（Lupinus angustifolius）的標記輔助選擇
7. Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness, uniformity and stability testing [O] . Arens, Paul, Mansilla, Carmen, Deinum, Dani?l, 2010

機譯：開發和評估與番茄抗病性相關的魯棒性分子標記，以進行獨特性，均勻性和穩定性測試

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Development and evaluation of robust molecular markers linked to disease resistance in tomato for distinctness uniformity and stability testing

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